Free WJEC GCSE Biology (3400) Exam Questions and Answers
WJEC GCSE Biology (3400) is WJEC's GCSE Biology qualification, specification 3400, taken in Wales. It is sat at Foundation or Higher tier. The questions here come from WJEC's published question papers from June 2017 to June 2025, plus its specimen papers, with answers and marking guidance taken from WJEC's own mark schemes. Written-answer questions let you work out your answer and then reveal what the mark scheme accepts; diagrams, graphs and source material appear with the questions that use them.
Looking for WJEC GCSE Biology (3400) exam dumps or ExamTopics questions? These practice questions cover the same ground with verified answers and explanations, a downloadable PDF and a full practice test, kept current as WJEC updates the exam.
Last updated: October 12, 2026
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Question #1
Image 1.1 shows a food web from an area of grassland. Use Image 1.1 to state the organisms which are present in the highest numbers.

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Correct answer: Grass(es) / producers
Explanation
One mark for grass(es) or producers.
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Question #2
Image 1.1 shows a food web from an area of grassland. Name the third stage (tertiary) consumers shown in the food web in Image 1.1. Give the reason for your choice. Name ____ Reason ____

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Correct answer: Owls. They feed on secondary consumers / carnivores (fourth trophic level).
Explanation
One mark for naming owls. One mark for the reason: they feed on secondary consumers / carnivores, or are at the fourth trophic level.
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Question #3
One food chain from the food web is shown below. The images are not to scale. grasses → insect → shrew → owl Table 1.2 shows the results of an investigation of the organisms in this food chain in the area of grassland habitat. Table 1.2 | Organism | Number of individuals counted | Mass of one individual (g) | Total biomass (g) | | shrew | 90 | 10 | ____ | | owl | 1 | 350 | ____ | | grasses | 10 000 | 2 | 20 000 | | insect | 3 000 | 4 | ____ | Complete Table 1.2 by calculating the total biomass for each of the organisms in this food chain. One has been done for you.

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Correct answer: shrew 900; owl 350; insect 12000
Explanation
Three correct values for 2 marks. One or two correct for 1 mark. None correct 0.
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Question #4
One food chain from the food web is shown below. The images are not to scale. grasses → insect → shrew → owl Table 1.2 shows the results of an investigation of the organisms in this food chain in the area of grassland habitat. Table 1.2 | Organism | Number of individuals counted | Mass of one individual (g) | Total biomass (g) | | shrew | 90 | 10 | ____ | | owl | 1 | 350 | ____ | | grasses | 10 000 | 2 | 20 000 | | insect | 3 000 | 4 | ____ | Use Table 1.2 to complete the pyramid of numbers in Image 1.3 by writing the names of the organisms in this food chain and the numbers of each.

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Correct answer: Owl 1; Shrew 90; Insect 3000; Grasses 10000 (owl at top, grasses at bottom)
Explanation
All four names and numbers must be in the correct positions for the mark.
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Question #5
One food chain from the food web is shown below. The images are not to scale. grasses → insect → shrew → owl Table 1.2 shows the results of an investigation of the organisms in this food chain in the area of grassland habitat. Table 1.2 | Organism | Number of individuals counted | Mass of one individual (g) | Total biomass (g) | | shrew | 90 | 10 | ____ | | owl | 1 | 350 | ____ | | grasses | 10 000 | 2 | 20 000 | | insect | 3 000 | 4 | ____ | State one way in which energy can be lost from a food chain and explain how this loss of energy is shown in the pyramid.

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Correct answer: Energy is lost through respiration / waste materials / heat. Each layer has fewer organisms than the one before, so numbers get smaller going up the pyramid.
Explanation
One mark for a way energy is lost (respiration, waste materials, heat). One mark for explaining that each layer has fewer numbers than the one below / the pyramid narrows going up.
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Question #6
Image 2.1 shows a sample of human blood as seen through a light microscope. The image labels a phagocyte and a red blood cell. The drawing in Image 2.2 shows the cell membrane of the phagocyte. Label the cytoplasm in the outline of the phagocyte.

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Correct answer: 60 mm
Explanation
One mark for 60 mm.
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Question #7
Image 2.1 shows a sample of human blood as seen through a light microscope. The image labels a phagocyte and a red blood cell. The drawing in Image 2.2 shows the cell membrane of the phagocyte. Measure the width x–x in mm on the drawing. Width x–x = ____ mm

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Correct answer: 60 mm
Explanation
One mark for 60 mm.
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Question #8
Image 2.1 shows a sample of human blood as seen through a light microscope. The image labels a phagocyte and a red blood cell. The drawing in Image 2.2 shows the cell membrane of the phagocyte. The width of the actual cell is 0.012 mm. Calculate the magnification of the drawing using the equation below. magnification = (width x–x on drawing)/(width of the actual cell) magnification = × ____

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Correct answer: 5000
Explanation
60 ÷ 0.012 = 5000. Error carried forward from the measurement in part I.
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Question #9
Complete the table by selecting functions from those below. transports hormones carries oxygen defends against disease clots the blood controls temperature | Part of blood | Function | | red blood cell | ____ | | white blood cell | ____ | | platelets | ____ |
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Correct answer: Red blood cell: carries oxygen; White blood cell (phagocyte): defends against disease; Platelets: clots the blood
Explanation
One mark each: carries oxygen, defends against disease, clots the blood.
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Question #10
There are many types of blood cell, each with a particular function. They all come from one type of cell found in bone marrow through the process of differentiation. This is shown in Image 2.3. Choose the letter A-D to give the term used for the various types of blood cells.

Correct answer: B
Explanation
B – specialised.
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Discussion
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