Free OCR GCSE Biology B (Twenty First Century) (J257) Exam Questions and Answers
OCR GCSE Biology B (Twenty First Century) (J257) is OCR's GCSE Biology B (Twenty First Century) qualification, specification J257, taken in England. It is sat at Foundation or Higher tier. The questions here come from OCR's published question papers from June 2018 to June 2025, plus its specimen papers, with answers and marking guidance taken from OCR's own mark schemes. Written-answer questions let you work out your answer and then reveal what the mark scheme accepts; diagrams, graphs and source material appear with the questions that use them.
Candidates comparing OCR GCSE Biology B (Twenty First Century) (J257) exam dumps, ExamTopics and other practice tests use this page for the answers and explanations behind each question. Download the free PDF, then sit the timed exam simulation before booking with OCR.
Last updated: October 13, 2026
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Question #1
The eye is a sense organ. Each part of the eye is adapted to its specific function. The table describes the functions of different parts of the eye. Complete the table by writing the part of the eye that matches each description. Choose from the words below: ciliary muscle cornea iris lens | Part of the eye | Description | | ____ | A ring of tissue that changes size to alter the diameter of the pupil, to control the amount of light entering the eye. | | ____ | A thin layer of transparent tissue in front of the pupil which bends light as it enters the eye. | | ____ | A thick layer of transparent tissue behind the pupil which bends light so it focusses on the retina. | | ____ | Changes the thickness of the lens to focus light from far and near objects. |
Type your answer, then reveal.
Correct answer: Parts of the eye: iris, cornea, lens, ciliary muscle (matched to the descriptions in the table, in the order listed in the mark scheme: iris, cornea, lens, ciliary muscle).
Explanation
Three or four correct = 3 marks; two correct = 2 marks; one correct = 1 mark.
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Question #2
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | Calculate the mean pupil size. Mean pupil size = ____ mm
Type your answer, then reveal.
Correct answer: 4.6 mm
Explanation
If the answer is 4.6 mm award 2 marks. Otherwise: method mark for (4.0 + 3.8 + 6.0)/3 or 13.8/3; accuracy mark for 4.6 mm.
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Question #3
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | Amir reads an article that suggests the average pupil size in bright light should be in the range of 2 – 4 mm. Amir thinks one of his results is an anomalous result. Which result is most likely to be the anomalous result? Give a reason for your choice.
Type your answer, then reveal.
Correct answer: 6.0 mm (experiment 3), because it is far greater than the other two results / it is greater than the range stated.
Explanation
One mark for identifying 6.0 / experiment 3. One mark for the reason. Allow: outside the range; the other two results are only 0.2 mm different; too high compared to the other results; the other results are between 2–4 mm. Ignore: 'not closely related' or '6.0 is the greatest'.
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Question #4
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | What can Amir do to make his results more precise?
Type your answer, then reveal.
Correct answer: Repeat his experiment again.
Explanation
Ignore: do more experiments, repeat it on different people, another experiment, do it more than once.
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Question #5
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | To create dark conditions Amir asks his friend to put on sunglasses. Amir draws two diagrams (Fig. 1.1 and Fig. 1.2) to show how the pupil changes when the light conditions change. Fig. 1.2 is incomplete. Complete Fig. 1.2 to show the pupil in dark conditions.


Type your answer, then reveal.
Correct answer: The pupil is drawn bigger than in the first diagram (Fig. 1.1).
Explanation
One mark for a pupil drawn larger than in the first diagram.
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Question #6
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | The change in pupil size is an example of a reflex. Which statement best describes a reflex? Tick (✓) one box.
Correct answer: A
Explanation
More than one tick = 0 marks.
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Question #7
Amir is investigating what happens to pupil size when a person moves from an area of bright light to an area of darkness. He measures the size of his friend’s pupil in bright light. His results are shown in the table below. | Experiment number | Pupil size (mm) | | 1 | 4.0 | | 2 | 3.8 | | 3 | 6.0 | What name is given to the structure that transmits electrical impulses from the eye to the central nervous system? Put a ring around the correct answer.
Correct answer: D
Explanation
More than one ring = 0 marks.
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Question #8
Amir is reading a newspaper but the words look blurry. When he looks out the window he can see everything outside clearly. Explain to Amir why the words in the newspaper look blurry and explain how this defect could be corrected. Explanation ____ Correction ____
Type your answer, then reveal.
Correct answer: Amir is long sighted. Light rays do not meet at the retina (they are focused behind the retina). This can be corrected with glasses with convex lenses, which bend the light rays more so they focus on the retina.
Explanation
Any three from: Amir is long sighted (allow hyperopia/hypermetropia); light rays are not focused at the retina / are focused behind the retina (allow eyeball too short, lens too thin, light not refracted enough, lens cannot become round enough); use glasses with convex lenses; they make the light rays bend more / focus the light or image on the retina. Do not allow responses that refer to light reflecting in the eye.
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Question #9
DNA is a polymer made of nucleotides. A nucleotide is made of a sugar, a phosphate and a base. The diagram shows the structure of DNA. On the diagram label the location of a base.

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Correct answer: A base correctly labelled, with the label line touching a base (A, T, C or G) in the DNA diagram.
Explanation
The label line must clearly touch the relevant part of the molecule. Only one of the four types of base needs to be labelled. Allow A, T, C or G as the label.
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Question #10
DNA is a polymer made of nucleotides. A nucleotide is made of a sugar, a phosphate and a base. The diagram shows the structure of DNA. On the diagram label the location of the sugar and phosphate group.

Type your answer, then reveal.
Correct answer: Sugar and phosphate correctly labelled on the outer strand (backbone) of the DNA diagram.
Explanation
The label line must clearly touch the relevant part of the molecule (the sugar-phosphate backbone strands).
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