Free AQA GCSE Biology (8461) Exam Questions and Answers
AQA GCSE Biology (8461) is AQA's GCSE Biology qualification, specification 8461, taken in England. It is sat at Foundation or Higher tier. The questions here come from AQA's published question papers from June 2018 to June 2025, plus its specimen papers, with answers and marking guidance taken from AQA's own mark schemes. Written-answer questions let you work out your answer and then reveal what the mark scheme accepts; diagrams, graphs and source material appear with the questions that use them.
Looking for AQA GCSE Biology (8461) exam dumps or ExamTopics questions? These practice questions cover the same ground with verified answers and explanations, a downloadable PDF and a full practice test, kept current as AQA updates the exam.
Last updated: October 14, 2026
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Question #1
HIV (Human Immunodeficiency Virus) is a pathogen. How is HIV spread from one person to another person? Tick (✓) one box.
Correct answer: B
Explanation
One mark for sexual intercourse.
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Question #2
HIV (Human Immunodeficiency Virus) is a pathogen. Table 1 shows information about new cases of HIV diagnosed in the UK. Describe what happened to the number of new cases of HIV from 2010 to 2018.

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Correct answer: The number of new cases increased at first, then decreased.
Explanation
Two marks: one for increased (at first) and one for (then) decreased. Ignore numbers unqualified. Do not accept an implication of an overall increase. If no other mark is awarded, allow (overall) decrease for 1 mark.
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Question #3
HIV (Human Immunodeficiency Virus) is a pathogen. What could cause a decrease in the number of new HIV cases in the future? Tick (✓) one box.
Correct answer: C
Explanation
One mark for better education on how to prevent the spread of HIV.
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Question #4
HIV (Human Immunodeficiency Virus) is a pathogen. Scientists have been working to produce a vaccine for HIV for many years. How could a vaccine work to prevent a person being infected with HIV? Write the stages A, B, C, D and E in the correct order. The first stage has been completed for you. A Antibodies attach to the inactive virus. B Antibodies destroy the inactive virus. C An inactive form of the virus is injected into the body. D If the active virus enters the body, antibodies are produced quickly. E White blood cells produce antibodies to the inactive virus.
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Correct answer: (C) → E → A → B → D
Explanation
Three marks for the fully correct order C → E → A → B → D (C is given as the first stage). Allow 1 mark for the E → A link, 1 mark for the A → B link, and 1 mark for the B → D link. If no other mark is awarded, allow 1 mark for the answer C → E → B → A → D.
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Question #5
HIV (Human Immunodeficiency Virus) is a pathogen. When scientists produce a vaccine for a disease the vaccine is tested on live animals. What is the next stage in testing the vaccine? Tick (✓) one box.
Correct answer: B
Explanation
One mark for testing on healthy volunteers.
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Question #6
HIV (Human Immunodeficiency Virus) is a pathogen. A vaccine for HIV is important because it is difficult to develop safe drugs to destroy viruses. Why is it difficult to develop safe drugs to destroy viruses? Tick (✓) one box.
Correct answer: A
Explanation
One mark for this option only.
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Question #7
HIV (Human Immunodeficiency Virus) is a pathogen. Some drugs originated from plants. Draw one line from each drug to the plant the drug originated from. Drug: Aspirin, Digitalis Plant the drug originated from: Foxglove, Rose, Tobacco, Willow






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Correct answer: Aspirin – Willow; Digitalis – Foxglove
Explanation
One mark for each correct line. Do not accept more than one line from a box on the left. (Line positions inferred from standard knowledge of the drug origins; the scheme page shows 2 marks.)
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Question #8
Figure 1 shows animal cells. Some of the cells are dividing by mitosis for growth and repair. What fraction of the cells in Figure 1 is dividing by mitosis? Tick (✓) one box.

Correct answer: B
Explanation
One mark for 1/4.
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Question #9
Figure 1 shows animal cells. Some of the cells are dividing by mitosis for growth and repair. The cells which are not dividing in Figure 1 each contain 10 chromosomes. One of these cells divides by mitosis to produce two new cells. How many chromosomes will each new cell contain after mitosis? Tick (✓) one box.

Correct answer: B
Explanation
One mark for 10 chromosomes.
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Question #10
Figure 1 shows animal cells. Some of the cells are dividing by mitosis for growth and repair. Cells divide in a series of stages called the cell cycle. Complete the sentences. Choose answers from the box. contracts, divides, grows, reacts, relaxes, replicates Before mitosis occurs, the cell ____. The genetic material in the cell doubles when the DNA ____. After the chromosomes have been pulled to each end of the cell, the cytoplasm ____.


Type your answer, then reveal.
Correct answer: Before mitosis occurs, the cell grows. The genetic material replicates. After mitosis the cell divides.
Explanation
Words must be in this order: grows, replicates, divides. One mark each.
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